灰木相思离体培养体系的构建

Optimum conditions for rapid propagation with clones of Acacia implexa

灰木相思Acacia implexa是南方优良的多用途造林树种,但种子繁殖性状分离严重,试图通过组织培养找到新的繁育途径。通过对灰木相思成年优树幼嫩的带腋芽茎段的离体培养试验,探讨了灰木相思茎段不定芽分化、继代增殖和壮苗生根的最佳培养条件。采用正交试验对防止培养过程中出现玻璃化苗的方法进行了探讨。结果表明:适宜灰木相思不定芽诱导的培养基配方为MS(Murashige and Skoog)+1.0 mg.L-16-苄氨基嘌呤(6-BA)+0.2 mg.L-1萘乙酸(NAA)+1.0 mg.L-1玉米素(ZT),适宜继代培养的培养基为改良1/2 MS(NH4+∶NO3-=1∶2)+1.0 mg.L-16-BA+0.05 mg.L-1NAA,适宜进行壮苗生根的培养基配方为改良1/2MS+0.1 mg.L-1NAA,移栽后成活率可达70%。

Acacia implexa is an excellent muhipurpose tree species in South China, however seed propagation will cause segregation of inheritable characteristics and then, limit its utilization. This study looked at the use of tissue euhure to clone trees with three plant growth regulators （6-BA; NAA, ZT） in different levels, and appropriate methods for possible ways to prevent vitrification of adventitious buds by orthogonal experiment L9 （34） . Results of experiments on micropropagation of young stem sections for selected adult trees of A. implexa showed that a suitable medium for adventitious bud differentiation of the stem sections with axillary buds was： MS （Murashige and Skoog＇s medium） ＋ 1.0 mg·L^-1 BA （6-benzyladenine） ＋ 0.2 mg·L^-1 NAA （a-naphthalene acetic acid ） ＋ 1.0 mg·L^-1 ZT （zeatin）, a suitable medium for adventitious bud multiplication was： 1/2 MS （NH4^＋ ：NO3^- = 1 ：2） ＋ 1.0 mg·L^-1 6-BA ＋ 0.05 mg·L^-1 NAA, and a suitable medium for improving roots was： 1/2 MS ＋ 0.1 mg·L^-1 NAA. After transplanting, over 70% of the tube plantlets survived. [Ch, 3 tab. 11 ref. ]