多壁碳纳米管修饰酶电极测定马拉硫磷

Determination of Malathion Based on Carbon Nanotubes Modified Electrode Incorporating Acetylcholinesterase

应用化学交联法将巴西日圆线虫乙酰胆碱酯酶（AChE）固定于多壁碳纳米管修饰的玻碳电极表面，制备用于有机磷农药检测的电化学生物传感器．研究了巯基胆碱（TCh）在该修饰电极上的电化学行为．优化实验条件得出，响应电流与马拉硫磷的浓度在6．0×10^-10～6．0×10^-9mol·L^-1范围内呈良好的线性关系，检出限为1．0×10^-10mol·L^-1．测定了湖水中马拉硫磷的含量，回收率为97％～105％．

A biosensor for determination of organophosphorus pesticide based on the acetylcholinesterase and multi-wall carbon nanotubes modified electrode is fabricated. Under the optimal conditions, the chronoampero- metric current at ＋ 0.70V （vs. SCE） increased linearly with the concentration of malathion in the range from 6.0 × 10^-10 to 6.0 × 10^-9 mol · L^-1 with a detection limit of 1.0 × 10^ -10 mol . L^-1. The recoveries of malathion in lake water is 97% to 105% determinated by this method.