大菱鲆(Scophthalmus maximus)一种CXC趋化因子的克隆及其表达特征

Cloning,characterization and expression analysis of a CXC chemokine from turbot (Scophthalmus maximus)

从大菱鲆脾脏cDNA文库中筛选到一种CXC趋化因子。它的全长cDNA含有一个81bp的5’UTR,一个414bp的开放阅读框和一个449bp的3’UTR。开放阅读框编码了137个氨基酸残基。经过PCR扩增发现,该CXC趋化因子基因内含有4个外显子和3个内含子。RT-PCR分析表明,大菱鲆CXC趋化因子在正常脾脏和头肾中表达强烈;在胚胎的不同发育时期,大菱鲆CXC趋化因子从体节期开始才有微弱的表达。大菱鲆胚胎细胞(TEC)在用鳗弧菌感染后6h也有强烈的表达。这些结果表明该CXC趋化因子在大菱鲆免疫应答中起着重要作用。

A turbot CXC chemokine was screened from a turbot spleen cDNA library. The complete cDNA of the turbot CXC chemokine contains an 81bp 5＇ UTR, a 414 bp open reading frame （ORF） encoding 137 amino acids and a 449 bp 3＇ UTR. Four exons and three introns were identified in the turbot CXC chemokine gene. RT-PCR demonstrated that the turbot CXC chemokine was expressed highly in spleen and kidney from turbot adult, and during the early stages of embryo development after fertilization, a low level of expression of the turbot CXC chemokine was firstly observed at somites stage. Furthermore, the expression of CXC chemokine was dramatically increased after challenge in turbot embryonic cells （TEC）. These results indicate that the turbot CXC chemokine plays an important role in turbot immune response.