血管内皮生长因子单抗对大鼠肝缺血再灌注损伤的影响

Effect of vascular endothelial growth factor monoclonal antibody on hepatic ischemia-reperfusion injury in rats

目的研究再灌注期使用血管内皮生长因子单抗(Vascular endothelial growth factor monoclonal antibody,简称VEGFmAb)对大鼠肝缺血再灌注损伤的影响。方法将SD大鼠18只分为两组(每组n=9)。C组:实验对照组,常规行肝缺血再灌注损伤实验;V组:VEGFmAb处理组:在再灌注期使用血管内皮生长因子单抗。于术后2h、24h分别取血测肝功能,术后24h切取缺血肝叶检测热休克蛋白(heat shock protein 70,简称HSP70)RT-PCR表达、肝病理组织学改变,观察生存状态1周。结果与C组比较,V组HSP70的RT-PCR表达未见明显改变;V组ALT、AST在术后明显下降,肝病理组织学改变有所减轻,1周生存率提高(55.56%vs0)。结论再灌注期使用VEGFmAb有助于减轻大鼠对缺血再灌注损伤,改善预后。

Objective To investigate the effect of vascular endothelial growth factor monoclonal antibody （VEGF mAb） on hepatic ischemia-reperfusion injury in rats. Methods 18 SD rats were divided into two groups. Routine hepatic ischemia-reperfusion injury experiment was performed as control group （group C） and VEGFmAb were used during reperfusion period in group and VEGFmAb （group V）. Blood were collected to examine liver function 2 hour and 24 hour ofter- operation. Ischemic liver-lobe were resected to detect the RT-PCR expression of heat shock protein 70 （HSP70） and pathological change. Survival status was observed in all rats for one week. Results RTPCR expression of HSP-70 was no obvious differece compared with group C. ALT and AST were declined evidently after-operation. Pathological damage of liver were relieved and one week survival rate was increased （55.56% vs 0）. Conclusion The use of VEGFm Ab in the reperfusion period would contribute to relief of hepatic ischemia-reperfusion injury and it may inprove the prognosis.