野生直立百部的化学成分

Chemical constituents of the roots of wild Stemona sessilifolia

目的：研究采集于江苏句容宝华山的野生直立百部Stemona sessilifolia（Miq．）Miq．的化学成分。方法：用75％乙醇回流提取，氯仿和正丁醇萃取，采用大孔树脂、反复硅胶柱色谱法和重结晶等方法分离纯化，根据理化性质和光谱数据鉴定化合物的结构。结果：从直立百部的根中分离得到10个化合物，分别鉴定为：7-甲氧基-3-甲基-2，5-二羟基-9，10-二氢菲（7-methoxy-3-methyl-2，5-dihydroxy-9，10-dihydrophenanthrene，1）、stilbostemin D（2）、原百部碱（protosteronine，3）、原百部次碱（protostemotinine，4）、stemospironine（5）、左旋丁香树脂酚葡萄糖苷[（-）-syringaresinol-4-O-β-D-glucopyranoside，6]、苯甲酸（7）、香草酸（8）、胡萝卜苷（9）和豆甾醇（10）。结论：化合物1为新化合物，化合物6首次从该属植物中分离得到，化合物7-10为首次从该植物中分离得到。

Aim： To study the chemical constituents of the roots of wild Stemona sessilifolia （Miq.） Miq. collected in Baohua Mountain, Jurong City, Jiangsu Province. Methods： The roots of wild S. sessilifolia were extracted with 75% EtOH and partitioned by CHCl3 and n-BuOH . The compounds were separated and purified by column chromatography with silica gel and macroporous resin, and their structures were identified by spectroscopic analyses. Results： Ten compounds were isolated from the root of S. sessilifolia in this study, i. e., one new dihydrophenanthrene named 7-methoxy-3-methyl-2,5-dihydroxy- 9, 10-dihydrophenanthrene （1）, and 9 of known compounds ： stilbostemin D （2）, protostemonine （3）, protostemotinine （4）, stemospironine （5）, （ - ）-syringaresinol-4- O-β-D-glucopyranoside （6）, benzoic acid （7）, vanillic acid （8）, daucosterol （9）, and stigmasterol （10）. Conclusion： Compound 1 is a new compound, compound 6 is isolated from the genus for the first time, and compounds 7 - 10 are isolated from the species for the first time.