摘要
目的:研究影响产气肠杆菌EAM-Z1的嘧啶核苷磷酸化酶(PyNPase)酶活力的因素。方法:分别考察添加底物、金属离子、速效碳源和磷酸盐对菌体浓度及PyNPase活力的影响。结果:加入4 g/L胞苷,菌体的量增加了19.30%,综合考虑对菌体量和比酶活,加入3 g/L胞苷可使PyNPase活力增加10.46%;4 mg/L Ca2+使菌体量增加了10.90%,PyNPase活力增加了30.25%;低浓度的速效碳源可以增加菌体浓度,但均使PyNPase的活力降低;1 g/L磷酸盐使PyNPase活力增加了17.40%。结论:底物诱导、金属离子、速效碳源和磷酸盐均对PyNPase的活力有一定的影响。
Aim:To study the factors affecting the enzymatic activity of pyrimidine nucleoside phosphorylase (PyNPase). Mothods:The influences of the substrate inducer, metalic ion, quick-carbon source and phosphate on the cell concentration and enzyme activity of PyNPase were investigated. Results: Cytidine (1 g/L) enhanced the cell concentration by 19.30%, and cytidine (3 g/L) enhanced the enzymatic activity by 10.46%. Ca^2+ (4 mg/L) can increase the cell concentration by 10.90% and increase the enzyme activity by 30.25%. Quick-carbon source of low concentration enhance the cell concentration but attenuated the enzymatic activity. Phosphate (1 g/L) increased the enzyme activity by 17.40%. Conclusion: Substrate inducer, metallic ion and phosphate are available measures to enhance the activity of PyNPase.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2007年第6期558-561,共4页
Journal of China Pharmaceutical University