肠道SIgA含量的变化与溃疡性结肠炎相关性的分析研究

Study of correlation between variation of intestinal SIgA content and ulcerative colitis

目的:在建立Wistar大鼠溃疡性结肠炎(UC)模型基础上,探讨肠道SIgA含量的变化与溃疡性结肠炎发病的相关性。方法:采用饮用DSS(Dextran sulfacte sodium)水溶液,建立UC动物模型,随机分为3组,即奥沙拉嗪治疗组(A组):2次/d奥沙拉嗪(Olsalazing)150 mg/kg灌胃;模型组(B组):UC模型大鼠每天饮用生理盐水;另设正常对照组(C组):每天饮用生理盐水。采用放射免疫分析法检测肠内容物分泌型IgA(SIgA)含量。结果:DSS所致大鼠UC模型简单易行与人类病变相类似。模型组SIgA含量较正常对照组明显降低(P<0.05),经奥沙拉嗪治疗后有明显回升,与模型组比较有显著差异(P<0.05),治疗组与正常对照组比较无差异(P>0.05)。结论:SIgA含量的变化与溃疡性结肠炎的发生有相关性的关系。

Objective： To establish a rat model of ulcerative colitis（UC） and to study the correlation between variation of intestinal SIgA content and pathogenesis of UC. Methods： Rat model of ulcerative colitis was established by oral administration of dextrane sulfate soctium（DSS） solution. These model rats were divided randomly into three groups. Treatment group（group A） was given olsalazing 150 mg/kg via intragastrie administration twice a day, animal model group（group 13） was given normal soctium（NS） and normal control group were given NS. Radioimmune assay was used to measure the level of SIgA in colonal contents. Results：Rat model of UC was successfully established and it resembled to human UC. The level of SIgA of group B was lower than that of the normal control group（P〈0. 05）. After treated with olsalazing, the content of SIgA increased significantly（P〈0. 05） and was similar to that of the normal control group. Conclusion：The variation of intestinal SIgA content is correlated with the genesis of UC.