摘要
以甜魔芋芽鞘为外植体,进行愈伤组织诱导、芽分化及植株再生的研究。结果表明:芽鞘在培养基MS+6-BA0.6 mg/L+NAA 0.1 mg/L+蔗糖30g/L上愈伤组织诱导率最高,达92.3%;芽分化最适培养基是MS+6-BA1.5 mg/L+NAA 0.5 mg/L+蔗糖30g/L,分化率达93.8%;切割后的芽在生根培养基1/2MS+NAA 0.1mg/L+蔗糖30g/L上,能100%形成完整植株。
The bud of Amorphophallus dunnii was used as explants for callus induction , bud differentiation and plant regen- eration. It showed that the maximal frequency was 92.3 % when cultured on MS ＋ 6 - BA0. 6mg/L ＋ NAA0. 1 mg/L ＋ sugar30g/L. The callus was differentiated to bud on MS ＋ 6 - BA 1.5mg/L ＋ NAA0. 5mg/L ＋ sugar30g/L with differ- ential rate being 93. 8 %. All adventitious buds rooted and formed whole plants in the rooting medium 1/2MS ＋ NAA 0. 1 mg/L ＋ sugar30g/L.
出处
《安徽农学通报》
2007年第22期36-37,共2页
Anhui Agricultural Science Bulletin
基金
湖北省"十一五"重大科技攻关项目(2006AA205A04)
关键词
甜魔芋
组织培养
再生植株
amorphophallus dunnii
tissue culture
regeneration plant
