空肠弯曲菌共同抗原的实验研究及初步应用

The experimental studies and primary application on of Campylobacter jejuni common antigen

用甘氨酸制备空肠弯曲菌酸提取物，能和５２种空肠弯曲菌分型血清发生交叉反应，提示空肠弯曲菌各型间存在共同抗原。ＳＤＳ－ＰＡＧＥ分析，显示该菌共同抗原分子量为３１０００。用其包被建立ＥＬＩＳＡ法检测２０３例腹泻病人血清抗体，与培养法比较，总符合率达９３．６％。

This article reported the acid extract from a strain of Campylobacter jejuni via glycine reacted to the typing serum of 52 Campylobacter jejuni.It suggested that there was a common antigen among the types of Campylobacters jejuni.Analysing the acid extract with SDS-PAGE system indicated the protein band of 31 000 molecular weight to be common antigen.Detecting the serum antibody from 203 diarrhoea patients by indirect ELISA in which the well coated with the common antigen,comparing with the cultivation,the total coincidence rate of 93 5%.