应用筑巢式RT-PCR检测BCR-ABL融合基因的探讨

Study of detecting BCR/ABL fusion gene by using nest RT PCR

应用筑巢式ＲＴ－ＰＣＲ检测２０例ＣＭＬ血或／及骨髓中ＢＣＲ／ＡＢＬ基因的表达，证实较普通ＲＴ－ＰＣＲ其为一种快速、敏感而准确的检测方法。同时以ＡＢＬ基因为ＲＮＡ及ｃＤＮＡ质量对照，有效地排除了因标本质量欠佳而致的假阴性或因标本污染所致的假阳性。我们还发现在ＣＭＬ中Ｐ２１０ＢＣＲ／ＡＢＬ的表达接近为１００％，且与化疗无明显相关。

Expression of BCR/ABL gene of peripheral blood or/and bone marrow was investigated in 20 CML patients by using nest reverse transcription polymerase chain reaction(RT PCR).In contrast to common RT PCR,it was confirmed to be a repid,sensitive and precise method for the BCR/ABL gene.Meanwhile ABL gene was tested as control for RNA and cDNA quality,so we could exclude effectively false negtive or positive because of worse cDNA qualities or contaminated samples.We also found that the expression of P210 BCR/ABL in CMLs was close to 100 percent and had no obvious relation to chemotherapy.