锆—偶氮胂Ⅲ络合物比色法测定尿草酸

Spectrophotometric determination of urinary oxalate with the Zironium ArsenazoⅢ complex

建立了尿草酸的比色测定方法。在强酸性条件下，锆与偶氮胂Ⅲ形成蓝色络合物，尿草酸置换该络合物中的偶氮胂Ⅲ而使吸光度降低，吸光度降低值与尿草酸浓度成正比。测定波长为６２０ｎｍ，线性范围０～０．１２０ｍｍｏｌ／Ｌ检测限０．０１１５ｍｍｏｌ／Ｌ，批内ＣＶ＝４．３％，日间ＣＶ＝６．１％，回收率９３．８％～１０６．２％，与变色酸比色法显著相关（ｒ＝０．９７１，Ｐ＜０．０１）。方法简单，稳定，快速，不需特殊仪器设备，适于临床尿常规分析。

Objective spectrophometric determination of urinary oxalate with the zironium arsenazoⅢ complex was developed.Ziroconium forms the comples with arsenazoⅢ with a absorbance peak at 620 nm,and then,arsenazoⅢ in the complex is replaced quantitatively by oxalate,which results in a decrease at 620 nm.The standard cure for oxalate was linear from 0 to 0 120 mmol/L. The within run CV and between run CV for urinary oxalate were 4.3% and 6.1%,respectively.It was shown that this method well matched with the comparative method with chromotropic acid.