人乳头瘤病毒DNA 6B/11、16、18在喉鳞癌组织中的表达

Detection of human papillomavirus genomes 6B/11 and 16/18 DNA in laryngeal carcinomas

为了证实人乳头瘤病毒（ＨＰＶ）亚型的感染与喉鳞癌的关系，采用原位杂交技术，荧光素标记ＨＰＶ６Ｂ／１１、１６、１８ＤＮＡ为探针，分别对３２例福尔马林固定、石蜡包埋的喉鳞癌进行ＨＰＶ６Ｂ／１１、１６、１８型检测。结果显示：ＨＰＶ１６型ＤＮＡ阳性１４例（４６％），ＨＰＶ１８型阳性９例（２８％），ＨＰＶ６Ｂ／１１阴性。提示：喉鳞癌的发生与ＨＰＶ１６、１８型感染有关。

The presence of human papillomavirus (HPV) genomes 6B/11 and 16/18 DNA was examined in 32 cases of laryngeal carcinomas with in situ hybridization (ISH),which had been fixed in formalin and embedded in paraffin.The results demonstrated that 14 (46%) cases showed positive reaction for HPV genome 16 and 9 (28%) cases for HPV genome 18,all cases negative reaction for HPV genome 6B/11.These results suggest that human papillomavirus genomes 16/18 infection might be closely associated with the development of laryngeal carcinomas.