硝化菌保藏特性及衰减动力学研究

Study on the Conservative Property and Decay Kinetic of Nitrite Oxidizing Bacteria

采用单因素试验设计及一阶指数衰减模型,对影响硝化菌保藏过程中的4个主要影响因素(温度、pH值、离子强度和渗透压调节剂)进行了研究,并建立了硝化菌的衰减动力学方程。研究发现,当温度为4℃,离子强度为0.035×3mol/kg,pH7.60时,硝化菌衰减指数由0.25降至0.013,半衰期由2d延长至53d。在低温环境(4℃)下,添加甘油可以较好地延缓硝化菌的衰减作用。

One factor at a time design and first order exponential model were applied to investigate the effects of the four major factors （temperature, ionic strength, pH and osmo-regnlator） in the preservation process of nitrite oxidization bacteria （NOB） , and a first order decay kinetics was introduced. The result showed that the anoxic decay rate of nitrifying bacteria reduced from 0.25 to 0. 015, the half life was extend to 53d at 4℃ , pH7.60, ionic strength 0.035mol/kg. The protecting effect of glycerin showed obviously better than that of other cryoprotectants at 4℃.