甲烷利用菌培养条件的优化及其初步应用

Optimization of Culture Conditions for a Methane Utilizing Strain and Its Preliminary Application

利用统计学实验设计对能够利用甲烷的假单胞菌菌株ME16主要培养条件进行了优化。以液体无机盐和甲烷气体作为培养基分别进行了温度、接种量、甲烷含量和培养pH对细菌生长影响的研究,并在此基础上,利用响应面法(RSM)分析优化了ME16菌株的主要培养条件,得到最佳培养条件为:温度29.4℃,接种量1.8%,甲烷含量25%。采用优化培养条件进行培养,细菌生物量增大0.8倍,达到稳定期的培养时间缩短了50h。该菌株初步应用于甲烷气体的脱除,脱除率达65.7%,表明该菌株能良好地脱除空气中的甲烷。

Statistic-based experimental design aeruginosa ME16 strain which is capable of utilizing was used to optimize the culture conditions for Pseudomonas methane. Effects of inoculum volume, temperature, methane content, and initial pH of media on cell growth were studied with the medium of mineral salt and methane gas, and the response surface methodology （RSM） was then used to optimize culture conditions. The optimum conditions were as follows： temperature of 29.4℃, inoculum volume of 1.8% and methane content of 25%. Under the optimized culture conditions, the ME16 production was increased by 0.8 times and its cultivation time was reduced. The removal of methane gas by ME16 was preliminary investigated, and the removal rate of methane gas reached 65.7%. The results indicated that the strain was capable of removing of methane.