川崎病患儿血清VEGF和NO水平变化及临床意义

The Change of Serum VEGF and NO and Their Clinical Significance in Patients with Kawasaki Disease

目的探讨川崎病(KD)患儿血清血管内皮生长因子(VEGF)和一氧化氮(NO)水平的变化及其临床意义。方法分别采用双抗体夹心酶联免疫吸附法(ELISA)、硝酸还原酶法测定38例川崎病患儿急性期、缓解期血清VEGF、NO水平,并与30例正常儿童对照。结果KD组急性期血清VEGF、NO水平明显高于缓解期和正常对照组(P均<0.01);缓解期显著下降,但与正常对照组比较,差异仍具有非常显著性意义(P<0.01)。CAL组血清VEGF、NO水平明显高于NCAL组(P<0.01、P<0.05)。急性期KD组患儿血清VEGF、NO水平呈显著的正相关(r=0.896,P<0.01)。结论VEGF与NO参与了KD血管炎的发生,且两者均可作为预测KD冠脉损伤的指标。

Objective To explore the change of serum vascular endothelial growth factor （VEGF）,nitric oxide（NO） levers in patients with Kawasaki disease（KD） and their Clinical significance. Methods Serum VEGF and NO were detected by the double antibody sandwich ELISA and the method of nitratase respectively in 38 patients with KD at their acute and remission stages, and compared with anther 30 normal children. Results The lever of serum VEGF, NO at the acute stage in KD group were significantly higher than those at the remission stage and the normal control group（P 〈0.01 ） ; At the remission stage in KD group , the lever of serum VEGF, NO were lower than those at the acute stage, but still higher than those in the normal control group（ P 〈 0.01 ）. The lever of serum VEGF,NO in the coronary artery lesions group were significantly higher than those in the non- coronary artery lesions （P 〈 0.01 ,P 〈 0.05）. At the acute stage , there was positive correlation between the lever of serum VEGF and NO （ r = 0. 896, P 〈0.01 ）. Conclusions The serum VEGF and NO are both involved in the pathogenesis of vasculitis in KD, and they may be used as markers to prognose the coronary artery lesions t in KD.