大鼠脑挫裂伤半暗带水肿与血-脑屏障破坏的关系

Relationship of edema and blood-brain barrier disruption in the penumbra area after rat focal brain contusion

目的研究大鼠局灶性脑挫裂伤半暗带的水肿变化与血-脑屏障(BBB)破坏的关系。方法将126只雄性SD大鼠随机分为3组:假手术组、挫裂伤组和给药组,采用Feeney法制作脑挫裂伤模型,给药组采用曲克芦丁脑蛋白水解物合剂腹腔给药,分别用伊文思蓝(EB)染色法和干湿法观察BBB的变化和脑组织的水肿情况,同时电镜观察超微结构。结果EB染色显示:挫裂伤组和给药组在伤后1 h EB开始漏出,6 h最严重。水含量测定结果显示:挫裂伤组和给药组伤后6 h水含量开始增加,72 h增至高峰。与挫裂伤组比较,给药组脑组织EB溢出量、水含量降低(P<0.05)。结论大鼠脑挫裂伤半暗带BBB通透性改变早于脑水肿的发生,提示BBB破坏可能是早期创伤性脑水肿的结构基础。曲克芦丁脑蛋白水解物合剂可通过改善BBB等多种途径治疗脑挫裂伤。

Objective To investigate the relationship of edema and blood-brain barrier （BBB） disruption in the penumbra area of rat focal brain contusion. Methods One hundred and twenty-six male SD rats were randomly divided into 3 groups： sham-operation group, contusion group and medication administration group. Brain contusion model was established using Feeney＇s method. In the medication administration group, the rats were injected intraperitoneally with troxerutin-brain protein hydrolysate mixture. Blood-brain barrier permeability and brain edema were evaluated by Evans blue extravasation and dry-wet weight method, and ultrastructure was observed under electromicroscope. Results In the contusion and medication administration groups, Evans blue extravasation was observed I h after trauma, peaked at the 6th hour. In the two groups, the brain water content increased fi＇om 6 h after trauma, and peaked at 72 h. Compared with the contusion group, the quantity of Evans blue extravasation and brain water content were lower in the medication administration group （P 〈0.05）. Conclusion After brain contusion, the change in BBB permeability is earlier than brain edema, which suggests that BBB disruption may be the base of early traumatic brain edema. Troxerutin-brain protein hydrolysate mixture is able to inhibit the occurrence and development of traumatic brain edema via improving the blood-brain barrier.