骨髓间充质干细胞在脑缺血模型大鼠脑组织中的迁徙、定居及组织修复作用

Migration,Colonization and Tissue-repairing Effect of Marrow Mesenchymal Stem Cells in Brain Tissues of Rats with Cerebral Ischemia-Reperfusion Injury

目的观察骨髓间充质干细胞(MSCs)移植后,在缺血性脑损伤大鼠脑组织中的迁徙、定居及组织修复作用。方法体外分离、纯化及培养MSCs。线栓法制备Wester大鼠脑缺血模型,经颈动脉移植DAPI标记的MSCs,通过激光共聚焦显微镜,分别在24h、5d及10d观察MSCs在脑组织内的迁徙及定居。采用三苯四氮唑活组织脑片染色方法,观察MSCs治疗对脑组织损伤的修复作用。结果MSCs经颈动脉移植后,在24h即出现在脑损伤区域血管内,第5天在血管周围组织内开始弥散,第10天在损伤区域可见广泛弥散。MSCs治疗20d后,脑片染色显示坏死区域减少。结论动脉移植MSCs后,MSCs首先出现在大鼠缺血性脑损伤区域血管内,然后在周围组织弥散,并可能参与损伤区血管及组织的修复。

Objective To observe the migration, colonization and tissue-repairing effect of transplanted marrow mesenchymal stem cells（MSCs） in the brain tissues of rats with cerebral ischemia-reperfusion injury. Methods MSCs were isolated from the bone marrow of suckling Wistar mice aged 5 d, purified and cultured in vitro. Establish Wistar rat model of cerebral ischemia-reperfusion injury by suture emboli method. Transplant DAPI-labeled MSCs into the brain tissues of model rats through carotid artery and observe their migration and colorization by laser confocal microscopy 24 h,5 d and 10 d later. Observe the repairing effect of MSCs on injury of brain tissue by triphenyhetrazolium staining. Results The diffusions of MSCs were observed in the blood vessels in injury regions 24 h, and in perivascular tissues 5 d after transplantation. However, extensive diffusion was observed in injury regions 10 d after transplantation. The triphenyhetrazolium staining of brain tissue section showed that the area of necrotic regions decreased significantly 20 d after transplantation. Conclusion The transplanted MSCs appeared in the blood vessels in injury regions at first then were diffused to perivascular tissues, and might be involved in the repair of blood vessels and tissues in injury region.