摘要
依据考马斯亮蓝G-250与蛋白质结合呈现蓝色,在595nm波长下的吸光度与蛋白质含量呈正比,制作出标准曲线。研究了最佳实验条件,成功地建立一个牛乳中蛋白质快速测定的方法,具有简单、快捷、易行等特点。结果表明该方法精密度高,RSD为1.70%,结果准确,相对误差较小,回收率98.2%￣100.3%。
A calibration curve can be drawn in accordance with the theory that the mixture of Coomassie Brilliant Blue G-250 with protein shows blue colour, and that the content of protein goes in direct ratio with the absorbance of Coomassie Brilliant Blue G-250 with the wavelength of 595nm. In the thesis, the determination of the optimum experimental conditions is focused on so as to find out a quick, simple and easy method of determining the content of protein in fresh milk. The results show that the method has a high degree of precision （RSD 1.7%） and little relative error with a recovery coefficient of 98.2%-100.3%.
出处
《饮料工业》
2007年第12期41-42,44,共3页
Beverage Industry
关键词
考马斯亮蓝
牛乳
蛋白质
Coomassie Brilliant Blue
fresh milk
protein
