摘要
目的建立龙芍利胆片中龙胆苦苷的含量测定方法。方法应用RP-HPLC法测定,选用C18色谱柱,流动相为甲醇-水(30∶70);流速1.0mL/min;检测波长270nm。柱温为25℃。结果线性关系良好,平均加样回收率为97.8%,RSD为1.7%(n=5)。结论方法简便、准确,可用于龙胆苦苷的质量控制。
Objective To establish the determination of gentiopicroside in Longshaolidan Tablet. Methods The RP-HPLC was carfled out on C18 column using methanol-water （30 ： 70）as a mobile phase. The detecting wavelength was set at 270nm.The flow rate was 1.0mL/min. The column temperature was at 25℃.Results The linear relationship was good.The average recovery was 97.8% and RSDwas 1.7%（n=6）. Conclusion The method is simple,reliable and suitable for the quality control of Longshaolidan Tablet.
出处
《特产研究》
2007年第4期61-62,共2页
Special Wild Economic Animal and Plant Research
