58例喉癌组织蛋白酶B表达及临床特征分析

Relationship between expression of cathepsin B and clinical feature of larynx cancer

目的探讨组织蛋白酶B(cathepsin B,CB)表达与喉癌发生、发展及浸润转移的关系。方法喉癌组58例肿瘤组织,其中临床分期Ⅰ期7例,Ⅱ期14例,Ⅲ期26例,Ⅳ期11例;病理分级高分化15例,中分化22例,低分化21例。另选择19例癌旁组织作对照。采用免疫组织化学SABC染色法和原位杂交法检测CB蛋白表达和mRNA水平情况。结果喉癌组CB蛋白定位于癌细胞胞浆,阳性率(44/58,75.9%)显著高于癌旁组(1/19,5.3%),淋巴转移组显著高于无转移组,不同临床分期阳性率显著不同。CB mRNA阳性率(35/58,60.3%)显著高于癌旁组(0/19),临床特征分析与CB蛋白相同。结论CB在喉癌组织中蛋白和mRNA水平均为高表达,CB表达与喉癌的发生、发展及浸润转移有关。

Objective To explore the expression regulation of cathepsin B in different period of larynx cancer. Methods Fiftyeight specimens of the patients sufferred from larynx cancer were included in this experiment. Among them,Tcases were in clinical stage I , 14 in stage Ⅱ ,26 in stage m and 11 in stage IV. Another 19 latero-tissue away from larynx cancer were included in the control group. The expression of cathepsin B was detected with the technology of immunohistochemistry staining and in situ hybridization. Results In the cancer group,the cathepsin B protein was expressed in the cell cytoplasm, the expressing rate of cathepsin B protein was markedly higher than that in the control group. The expressing rate of cathepsin B protein in the lymphatic metastasis group was higher than that in the no lymphatic metastasis group. In the different clinical stage, the expressing rates of cathepsin B protein were different. In the cancer group,the cathepsin B mRNA was expressed in the cell cytoplasm, the expressing rate of cathepsin B mRNA was markedly higher than that in the control group. Conclusion The abnormal high expression of cathepsin B protein and mRNA plays an important role in the occurrence of larynx cancer. The expression of cathepsin B has relationship with the progress and infihration of larynx cancer.