不同生态型同源四倍体和二倍体白菜品种的同功酶比较

COMPARISION OF ISOENZYMES BETWEEN AUTOTETRAPLOID AND DIPLOID CHINESE CABBAGE VARIETIES OF DIFFERENT ECOTYPES

测定了在１８℃的生长箱内培养的５个白菜同源四倍体及２个二倍体品种子叶期幼苗的过氧化物酶（ＰＥＲ）、酯酶（ＥＳＴ）及超氧化物歧化酶（ＳＯＤ）活性。结果显示，耐寒生态型四倍体品种的ＰＥＲ和ＥＳＴ比相应二倍体品种增加１～２条特有的酶带，ＳＯＤ谱带无显著的差异；耐热四倍体品种则相反，３种酶带皆较二倍体品种减少１或２条。对３种酶进行聚类分析的结果表明，相同生态型和染色体倍数相同的品种归为同类，与按表型分类的结果基本一致。

The study on isoenzymes of peroxydase (PER), esterase (EST) and SOD of the seedlings in the cotyledon stage of five autotetraploid and two diploid varieties planted in the culture box at 18 ℃ indicated that PER and EST isoenzyme bands of the cold resistant tetraploid varieties were one or two more than those of the corresponding diploid varieties yet their SOD isoenzyme bands were not notably different. In contrast, PER, EST and SOD isoenzyme bands in the heat resistant tetraploid varieties were one or two less than those of the diploid varieties. The cluster analysis of PER, EST and SOD isoenzymes showed that varieties of the same ecotype and multiple genome belonged to the same category, essentially consistent with classification of phenotype.