摘要
对影响鼠尾草离体再生的因素进行了研究,并以腋芽诱导率和平均每个外植体腋芽伸长数作为指标,对不同激素配比进行正交试验,筛选出了最佳的培养基组成.试验结果表明:在四种基因型中,粉萼鼠尾草的效果最好;鼠尾草腋芽诱导和伸长的最佳培养基组合为MS+ZT0.8 mg/L+KT0.2 mg/L+6-BA0.4 mg/L+NAA0.05 mg/L;适合于腋芽生根的不定根诱导培养基组合为1/2MS+IAA0.3-0.4 mg/L.
The facts affecting the plant regeneration of salvia are studied. Inducing rate of axillary and average of axillary buds of per explants are then calculated in orthogonal test to optimize combination of phytohormones for inducing and elongating axillary. It is indicated through the results that the best for inducing and elongating axillary is salvia farinacea among the four genotypes. It is also pointed out that the maximum rate of inducing axillary and the large number of elongating axillary per explants is obtained with MS ＋ ZT0. 8mg/L ＋ KT0. 2 mg/L ＋ 6 - BA0. 4 mg/L ＋ NAA0. 05 mg/L, and that elongated shoots are successfully rooted on 1/2MS ＋ IAA0. 3 - 0. 4 mg/L.
出处
《昆明理工大学学报（理工版）》
2007年第6期84-88,共5页
Journal of Kunming University of Science and Technology(Natural Science Edition)
基金
云南省自然科学基金(项目编号:2005C0023Q)
云南省教育厅青年科学研究基金(项目编号:04Y559B)
关键词
鼠尾草
组织培养
植株再生
正交实验
salvia
tissue culture
plant regeneration
orthogonal test
