光生物反应器中光强和Fe^(3+)浓度对铜绿微囊藻生长和毒素合成的影响

Effects of Light Intensity and Fe^(3+) Concentration on Microcystis aeruginosa Growth and Microcystin Production in a Photobioreactor

在2.5L光生物反应器中考察了光强、通气量等对铜绿微囊藻生长的影响,确定了最佳生长条件.在此条件下考察不同Fe3+浓度(0～500μmol/L)对铜绿微囊藻的生长、叶绿素及微囊藻毒素(MC-LR)含量的影响,同时考察光照强度对铜绿微囊藻产毒素的影响.实验结果表明,在低铁(Fe3+<0.1μmol/L)、缺铁(Fe3+<0.01μmol/L)及高铁(Fe3+>100μmol/L)环境下,微囊藻生长、叶绿素及毒素合成均受到抑制.生物量和叶绿素在Fe3+浓度为100μmol/L时含量最高,毒素在Fe3+浓度为10μmol/L、光强为30μmol/(m2-s)时含量最大.

The optimal conditions of microcystis aeruginosa cultured in a photobioreactor were established. The effects of light intensity and Fe^3＋ concentration on Microcystis aeruginosa growth and microcystin production in the photobioreactor were studied. The Fe^3＋ concentration in the culture medium changed between 0-500 μnol/L. The results showed that the cells growth and microcystin-LR production were both affected by changing Fe^3＋ concentration and light intensity. Biomass and chlorophyll were limited when Fe^3＋ concentration was below 0.1 μmol/L and reached the maximum when Fe^3＋ concentration was 100 μmol/L. Then they both dropped down when Fe^3＋ concentration was further increased. The maximum production of microcystin-LR was obtained at 10 μmol/L Fe^3＋ concentration. The effect of light intensity on microcystin-LR production was also studied under the optimal Fe^3＋ concentration. The results showed that the ootimal light intensity for microcvstin-LR production was 30 μmol/（m^2.s）.