摘要
Objective To investigate the therapeutic potential of tumor necrosis factor （TNF）-related apoptosis-inducing ligand （TRAIL）, a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors （DR4 and DR5） was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation （DNA ladder） and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced intemucleosomal DNA fragmentation （characteristic of apoptosis） and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.
Objective To investigate the therapeutic potential of tumor necrosis factor （TNF）-related apoptosis-inducing ligand （TRAIL）, a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors （DR4 and DR5） was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation （DNA ladder） and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced intemucleosomal DNA fragmentation （characteristic of apoptosis） and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.
基金
This research was supported by Jiangsu Projects for Postdoctoral Research Funds (No. 0601025B)
National Projects for Postdoctoral Research Funds (No. 20060390940).
