超微生物力学测量中的原子力显微镜技术

Micro-scale Biomechanics Measurements with Atomic Force Microscopy and the Concerned Techniques

原子力显微镜不仅可以在分子水平上对作用力进行测量,得到表面形状的图像,而且也可以用于高分子的解旋和空间构象的研究,此外也可研究样品表面的其他物理特性如压弹性、粘弹性特性的研究。文章首先阐明了微观力学表征的生物学意义,接着强调了原子力显微镜探针与样品间相互作用的类型及其在生物力学量测量中的原理和过程,之后分别介绍了原子力显微镜在细胞间黏附力、生物分子间相互作用、单分子力谱等方面的应用成果,最后结合原子力显微镜的应用现状对微观生物力的原子力显微镜深入研究进行了展望。

There are the tight relations between biomechanics and nearly all of the physical and chemical process in the biological life, and these mechanical parameters can provide enough proofs for the life science. In the past years, scientists have been trying to measure these parameters with different equipments and methods, in which the atomic foree microscopy may be the best and most successful equipment and techniques. As a novel tool in nanostructure of materials special in life science, atomic foree microscope runs a key role in the life process, and it can get the surface topographies of the sample and much more about the physical and mechanic properties. Firstly, demonstrate the meanings of the token for biomechanics and then stress on the type of the interaction between stylus and sample surface; secondly, introduce the application of AFM in the cell, biomolecules and single molecule. And end with the prospecting and discussions for the deep researches to biomechanics in micro-scale with atomic force microscope.