摘要
运用紫外光谱和荧光光谱技术研究咖啡因与胰α-淀粉酶的结合。反应类型为非竞争性抑制。Ki值为0.47×10-2mol/L。根据Stern-Volmer方程,计算了咖啡因对胰α-淀粉酶的表观淬灭常数,推测其荧光影响可能属于静态淬灭机制。
By using ultraviolet and fluorescent spectrometry, we study the relation between caffeine and pancreatic amylase. The reaction in this research was investigated. The Ki was 0.47 × 10^-2 mol/L. According to the Stern-Volmer equations ,we compute the expressing quench parameter of pancreatic amylase influenced by caffeine. We guessed that the underlying mechanism belong to static quench.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第6期1016-1019,共4页
Natural Product Research and Development
关键词
胰α-淀粉酶
咖啡因
抑制作用
紫外光谱
荧光光谱
pancreatic amylase
caffeine
prohibitive function
ultraviolet spectra
fluorescent spectra
