红葡萄酒对大鼠肝脏抗氧化酶和脂质过氧化的影响

Effect of Red Wine Intake on the Activities of Antioxidant Enzymes and Lipid Peroxidantion of Rat Liver

选用雄性SD大鼠,分别灌胃红葡萄酒、酒精及水。实验90 d中每隔30 d处死一批动物,测定大鼠肝脏匀浆组织中的超氧化物歧化酶(Superoxide dismutase SOD)、过氧化氢酶(Catalase CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase GSH-Px)活性和脂质过氧化产物丙二醛(Malondialdehyde MDA)含量变化。观察摄入红葡萄酒后大鼠肝脏抗氧化酶活性变化及对肝脏脂质过氧化的影响。结果表明,红葡萄酒能提高SOD活性,且SOD活性与灌胃时间、剂量有一定关系;长期红葡萄酒和酒精摄入可诱导CAT活性增强,加剧肝脏的脂质过氧化(LPO)作用;红葡萄酒组、酒精组0.63、1.25 g/kg剂量GSH-Px活性均明显升高(P<0.05),酒精组1.88 g/kg剂量有极显著性差异(P<0.01);试验初期,红葡萄酒大剂量显著降低肝脏中MDA的含量。试验中期,红葡萄酒中大剂量显著降低MDA含量。试验末期,红葡萄酒大剂量和酒精中大剂量显著升高肝脏中MDA含量。

Male Sprague Dawley rats were divided into 3 groups, 12.5% red wine, 12.5% ethanol （ the dose of 0.63, 1.25,1.88 g/kg body weight） and water control group. The activities of SOD, CAT, GSH-Px and MDA content were measured at 30,60 and 90 clays of the experiment respectively. The result showed the consumption of red wine by rats resuited in significant increases in the activities of SOD, CAT and GSH-Px but not the body weight after 90 days compared with the control group; the consumption of alcohol increased the activities of CAT and GSH-Px. The contents of MDA in the rats of red wine group were decreased significantly after 90 d. At the same time, the results examined showed： if given the same drinking level, the liver damages in rats of red wine group were much less than those of alcohol group, what＇ s more, the liver conditions in rats of red wine group were improved.