羊栖菜褐藻糖胶部分酸水解产物中寡糖的结构分析

Structural Investigation of Oligosaccharides in Partial Acid Hydrolyzed Products of Fucoidan Isolated from Hizikia fusiforme

研究了羊栖菜褐藻糖胶DSF32部分酸水解小分子产物中寡糖的结构。DSF32经0.5 mol/L三氟乙酸(TFA)部分酸水解,水解液用截留分子量为3500 Da的透析袋进行透析,然后用D201柱将透过液分为中性糖部分(05N)和酸性糖部分(05A)。采用液质联用和甲基化分析研究了它们的结构,结果表明,05A可能存在以下寡糖:→4)G lcA(1→2)Hex(1→,→2)Hex(1→4)G lcA(1→2)Hex(1→,→4)G lcA(1→2)Hex(1→3)Fuc(1→。05N的一个组分05N-3的非还原末端是Hex(1→,还原末端是2→)Hex,寡糖中间的糖苷键类型主要是1,6,另外还有少量的1,4和1,2,6连接方式。

DSF32, a fraction of fucoidan isolated from Hizikia fusiforme, was partially hydrolyzed by 0.5 mol/L trifluoroacetic acid. The hydrolyzed product was dialyzed using a dialyzer with a Mw cutoff of 3500 Da. The permeance of dialysis was separated into two parts of neutral sugars （05N） and acid sugars （05A） by D201 column. The structures of 05N and 05A were studied by HPLC/MS and methylation analysis. The results showed that 05A may contain the following oligosaccharides ：→4） GlcA（1→2） Hex（1→,→2） Hex（1→4） GlcA（1→2） Hex（ 1→ and →4） GlcA（1→2） Hex（ 1→3 ） Fuc（ 1→. 05N-3, a component of 05N,has a non-reducing end of Hex （1 → and a reducing end of 2→）Hex, and the types of glycosidic bonds in oligosaccharides are mainly 1,6 plus a small amount of 1,4 and 1,2,6.