夏枯草多糖的分离、纯化及结构初步分析

Purification and Structure Analysis of Polysaccharides from Prunella vulgaris Linn

采用100℃热水提取,得到夏枯草粗多糖。经阴离子交换柱层析、凝胶过滤柱层析对粗多糖进行纯化分级,通过高效凝胶过滤色谱测得了其主要组分PLS3的重均分子量为8.3×10-5Da。对PLS3进行了红外分析,测定了其初步结构;并分别以气相色谱和柱前衍生化高效液相色谱法测定了粗多糖和PLS3的单糖组成,发现两者所含单糖种类一致,都为半乳糖、葡萄糖、甘露糖、木糖、阿拉伯糖、鼠李糖,但两种物质的单糖组成相对摩尔百分比有所差异。

Polysaccharide from Prunella vulgaris Linn was extracted with water at 100 ℃. It was fractionated by anionexchange chromatography and gel filtration chromatography. The results showed that weight average molecular weight of the major portion PLS3 was 8.3 × 10^-5 Da by HPGPC. The primary structure of PLS3 was determined from its IR spectrum. The pro-column derivatization HPLC and GC analysis showed that the polysaccharide from P. vulgaris Linn and PLS3 were both composed of galactose, glucose, mannouse, xylose, arabinose and rhamnose without uronic acid. But the moler percentages of monosaccharides are different.