南方红豆杉和东北红豆杉中的单体化合物对乳腺癌细胞增殖的影响

Effect of the Compounds from Taxus chinensis var. mairei and T. cuspidata on the Proliferation of Human Breast Cancer Cells

本研究采用MTT法，研究南方红豆杉和东北红豆杉中的10种不同结构类型的单体化合物对乳腺癌MCF-7细胞增殖的影响。结果表明，化合物1-10（10^-9-10^-5mol/L）处理MCF-7细胞48和72 h后，仅化合物4在10^-7、10^-6和10^-5mol/L浓度对MCF-7细胞增殖均有明显抑制作用，抑制率分别为29.8%、46.4%、51.8%和43.6%、61.2%、63.2%，与紫杉醇抑制细胞增殖的活性相近，且在24-72 h范围内具有时间依赖性；化合物2仅在10^-5mol/L具有明显抑制细胞增殖的作用，抑制率为44.4%和49.6%。因此，10种不同结构类型的单体化合物中，仅baccatin Ⅲ类化合物2、4对MCF-7细胞增殖具有较强的抑制作用，其中化合物4作用最强，活性与紫杉醇相近。

MTT assays were used to study the effects of 10 compounds from Taxus chinensis var. mairei and T. cuspidata on the proliferation of MCF-7 human breast cancer cultured cells in vitro in this study. After treated with compounds （ 1- 10） （10^-9-10^-5 mol/L） for 48 h and 72 h,compound 4 produced a significant inhibition effect on MCF-7 cells at 10^-7- 10^-5 mol/L （ P 〈 0.01 ）, and the inhibitory rate was 29.8% ,46.4% ,51.8% or 43.6% ,61.2% ,63.2%, respectively, nearly similar to taxol at the same concentration. During the period of 24-72 h, the inhibition effect was time-dependent. Compound 2 showed a significant inhibition on MCF-7 cells only at 10.5 mol/L （P 〈 0.01 ）, and the inhibitory rate was 44.4% and 49.6%. Consequently, compounds 2 and 4 ,the analogues of bacctin Ⅲ, inhibited the proliferation of MCF-7 breast cancer cells significantly, and compound 4 had the higher activity among them, nearly similar to taxol.