RP-HPLC法测定银黄冲剂中黄芩苷在大鼠体内的血药浓度及药代动力学研究

Determination of Baicalin of Yinhuan Granules in Rat Plasma by HPLC and Study on Its Pharmacokinetics

采用高效液相色谱法测定银黄冲剂中黄芩苷在大鼠血浆中的浓度，并研究其药代动力学。色谱柱为Diamonsil^TMC18（250 mm×4.6 mm，5μm）柱，流动相：甲醇-磷酸二氢钠缓冲液（pH 2.6），体积比为52：48，检测波长275 nm，流速：1.0 mL/min。结果血浆中黄芩苷在0.05-20μg/mL范围呈良好的线性关系，r^2=0.9997，最低定量限为6 ng/mL。大鼠按1.6 g/kg灌胃银黄冲剂后，黄芩苷的血药浓度时间曲线符合口服吸收有滞后时间的二房室模型，主要药动学参数为t1/2：0.17 h，Vd：2.65 L，K12：5.36/h，K21：0.71/h，Ke：4.25/h，AUC：2.01μg·h/mL。HPLC测定黄芩苷的血药浓度操作便捷，灵敏度高，适用于黄芩苷的药代动力学研究。

This paper established HPLC method for determination of baiealin of Yinhuan granules in rat plasma and studied its pharmacokinetics. The separation was performed on Diamonsil^TM C18 column （250 mm×4.6 mm,5 μm）, using methanol-sodium dihydrogen phosphate solution （pH 2.6）, （52：48, v：v） as mobile phase, the flow rate was at 1.0 mL/ min,the detection wavelength was at 275 nm. The liner range of baicalin was 0.05 -20 μg/mL,r^2 = 0.9997 ,the LLOQ was 6 ng/mL. The plasma concentration-time curve of baicalin was fitted with a two-compartment open model with a lag time,with the following pharmacokinctic parameters t1/2：0.17 h,Vd： 2.65 L,K12：5.36/h,K21 ： 0.71/h,Ke： 4.25/ h, AUC： 2.01 μg · h/mL, respectively. The improved analytical method for baicalin was found to be rapid, sensitive and suitable for application in pharmacokinetic studies.