摘要
本文采用超声技术提取车前草中可溶性膳食纤维;通过单因素及L9(34)正交实验获得提取最佳工艺,即用固液比1:30,pH为4的0.2 M乙酸-乙酸钠缓冲液提取,提取时间为20 min。车前草水溶性膳食纤维对.OH-自由基有较强的清除能力,其IC50为0.323 mg/mL;对.O2-和DPPH的最高清除率分别为19.2%和13.7%。
Soluble dietary fiber was extracted by hot water from Plantago asiatica L. and prepared by ultrasonic technique. The optimal conditions were obtained by a series of mono-factor experiments and L9 (3^4) orthogonal test. The experiments showed that the optimal conditions were using 0.2 M acetic acid-acetic acid sodium buffering agent,pH 4 to distill;the liquid-solid ratio was 1:30;the extraction time was 20 rain. The soluble dietary fiber has a high scavenging activity on · OH^- free radical;IC50 is 0. 323 mg/mL. The · O2^- and DPPH free radical can be scavenged by Plantago asiatica L. soluble dietary fiber,the top scavenging ratio were 19.2% and 13.7% ,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第4期667-670,共4页
Natural Product Research and Development
基金
江西省教育厅课题(赣教技字[2005]52)
关键词
车前草
可溶性膳食纤维
超声技术
自由基
Plantago asiatica L.
soluble dietary fiber
ultrasonic technique
free radicals