车前草可溶性膳食纤维的提取及其对自由基清除能力的研究

The Extraction of Soluble Dietary Fiber from Plantago asiatica and Its Free Radicals Scavenging Activity

本文采用超声技术提取车前草中可溶性膳食纤维;通过单因素及L9(34)正交实验获得提取最佳工艺,即用固液比1:30,pH为4的0.2 M乙酸-乙酸钠缓冲液提取,提取时间为20 min。车前草水溶性膳食纤维对.OH-自由基有较强的清除能力,其IC50为0.323 mg/mL;对.O2-和DPPH的最高清除率分别为19.2%和13.7%。

Soluble dietary fiber was extracted by hot water from Plantago asiatica L. and prepared by ultrasonic technique. The optimal conditions were obtained by a series of mono-factor experiments and L9 （3^4） orthogonal test. The experiments showed that the optimal conditions were using 0.2 M acetic acid-acetic acid sodium buffering agent,pH 4 to distill;the liquid-solid ratio was 1：30;the extraction time was 20 rain. The soluble dietary fiber has a high scavenging activity on · OH^- free radical;IC50 is 0. 323 mg/mL. The · O2^- and DPPH free radical can be scavenged by Plantago asiatica L. soluble dietary fiber,the top scavenging ratio were 19.2% and 13.7% ,respectively.