HP-20树脂分离与纯化荷叶黄酮的技术研究

Study on Technology of Separation and Purification for Flavones in Lotus Leaf by HP-20

荷叶黄酮是荷叶中的主要活性成分之一,主要为黄酮醇和黄酮苷类,成为现代健康产品研究开发的热点。试验以高含量的荷叶黄酮为样品,比较X-5、HP-20,NKA、AB-8及HPD-100几种大孔吸附树脂的吸附效果,结果显示HP-20分离效果最好,静态饱和吸附量可达68.90 mg/g树脂,解吸率达87.23%,动态饱和吸附7 mg/mL的荷叶黄酮的料液可达15 BV。系统优化了HP-20树脂柱分离纯化用70%的乙醇提取的荷叶醇提物,得到荷叶黄酮的分离纯化的技术参数。结果表明:上样浓度为200 mg/mL,流速为2 BV/h的洗脱速度时,40%的乙醇对荷叶黄酮的洗脱能力较强,经过一次分离黄酮含量可达62.06%,此梯度黄酮的得率可达81.40%,荷叶黄酮的总得率为98.17%,经过再分离,荷叶黄酮含量可提高到98.87%。

As the chief activity component,lotus flavones have been one of the hot points of healthy products research. The research compared the effect of 5 kinds of resins of X-5, HP-20,NKA, AB-8 and HPD-100. The results showed that HP-20 was the best one. Its static absorbtion was 68.90 mg/g resin. The rate of desorption was 87.23%. The research optimized the technique parameters systematically of HP-20 separating lotus flavones. The results showed that the saturated absorption of lotus flavones by HP-20 was 15 BV of lotus flavone of 7 mg/mL concentration. The concentration of lotus flavones could reach 62.06% desorbed by 40% ethanol for one times when the concentration of the added lotus flavones was 200 mg/mL and the speed of desorption was 2 BV/h. The rate of lotus flavones desorbed by 40% ethanol was 81.40% and the total rate of desorbed lotus flavones was 98.17%. The concentration of lotus flavones can reach to 98.87% if they are separated for several times.