应用着丝粒探针检测40例卵巢癌患者8号染色体数目的异常增多

Chromosome 8 Abnormal Was Detected in 40 Ovarian Carcinoma Specimens with DNA Fluorescence Probes of Certromere

目的确定卵巢癌中8号染色体数目异常改变的频率,分析此改变与卵巢癌临床参数之间的相关性。方法应用着丝粒探针通过荧光原位杂交技术对40例卵巢癌标本进行8号染色体数目检测。结果40例卵巢癌患者中23例(57。5%)出现8号染色体数目增多,其中8号染色体数目变化与各临床参数之间存在相关性,但无统计学意义。结论8号染色体数目数目异常增多是卵巢癌特征性遗传改变,有可能成为卵巢癌分子诊断的标志物。

Objective To determine the frequency of chromosome 8 abnormal alteration in ovarian carcinoma, and analyze the association between the alteration and clinical parametre of ovarian carcinoma. Methods Fluorescence in situ hybridization （FISH） and DNA probes for centromere 8 were used to examine the copy number of chromosome 8 in ovarian carcinoma samples from 40 patiens. Results Tweenty three of 40 samples（57.5% ） had a gain in copy number of chromosome 8, and the alterations in chromosome 8 number were not statistically significantly associated with clinical parameter of these patients. Conclusion The gain of chromosome 8 is the characteristic genetic alteration in ovarian cancer tissue campared with normal tissue, and may be taken as the biomaker for tumor dignosis and progressional prediction in ovarian carcinoma..