生物催化肾上腺酮不对称合成R-肾上腺素

Asymmetric biosynthesis of R-adrenalin from corticosterone

研究了生物不对称还原肾上腺酮生成单一对映体R-肾上腺素.以肾上腺酮为前体,从土壤中筛选到一株对肾上腺酮具有较高立体选择性催化的菌株S289,以其为出发菌株,依次进行紫外和亚硝酸诱变,得到R-肾上腺素高转化菌株UN-78.确定了最佳转化条件,在初始底物浓度为10 mmol/L、摇床转速180 r/m in、温度30℃和pH 6.5,R-肾上腺素得率达56.2%,对映体过量值e达到99.8%.

Asymmetric biosynthesis of R -adrenalin was studied in this paper. A new strain S289 was isolated from soil. This strain can synthesize R - adrenalin by asymmetric bioreduetion of eortieosterone, and it was selected as original strain. The mutant strain UN -78 with effective transforming ability and high enantioseleetivity was obtained via ultraviolet and nitrous acid mutagenesis. After the optimization of transformation, the final yield and enantiomerie excess of R - adrenaline were 56.2% and 99.8% respectively, when the initial substrate concentration was 10 mmol/L, rotation speed 180 r/min, temperature 32 ℃ and pH 6.5.