乌苏瑞宁的抗血栓作用

Antithrombotic effect of verussurinine from Veratrum nigrum var. ussuriense alkaloids

目的研究乌苏里藜芦总生物碱中乌苏瑞宁对大鼠的抗血栓作用，确证乌苏瑞宁是否为总碱中抗栓活性成分。方法应用电刺激诱发大鼠颈总动脉血栓形成模型和瘀血诱发大鼠下腔静脉血栓形成模型评价乌苏瑞宁的抗动、静脉血栓形成作用；采用Born’s法测定大鼠体内外血小板聚集率以研究乌苏瑞宁的抗血小板作用。结果大鼠iv5种不同剂量的乌苏瑞宁（1．25～20．00μg／kg）均能导致电刺激诱发的大鼠颈总动脉血栓形成所致的血管阻塞时间显著延长；亦能减少大鼠下腔静脉血栓的干质量；乌苏瑞宁在剂量为1．25～5．00μg／kg以及质量浓度为6．25～50μg／L时，能抑制大鼠体内外血小板聚集。结论乌苏瑞宁具有强大的抗大鼠动、静脉血栓形成作用和抗血小板作用。乌苏里藜芦总碱的抗血栓作用与其中的化学成分乌苏瑞宁有关。

Objective To study the effects of verussurinine （VSRN）, an alkaloid isolated from Veratrum nigrum var. ussuriense alkaloids （VnA）, against thrombosis and its platelet aggregation inhibitory activity in rats in order to find out whether VSRN is the antlthrombotic active ingredient of VnA. Methods The electrically induced rat carotid artery thrombosis and stasis-induced rat inferior vena cava thrombosis models were used to evaluate the antl-arterial and anti-venous thrombosis effect of VSRN, respectively. Born＇ s turbidimetric method was used to examine the in vivo and in vitro antiplatelet effect so as to investigate the antiplatelet aggregation of VSRN. Results In comparison with saline, VSRN in five different doses （1. 25-20. 00 μg/kg） showed significantly and dose-dependently prolonged occlusion time （OT） of carotid artery injured by electrical stimulation and reduced thrombus dry weight of inferior vena cava ligated for 4 h to cause stasis. Platelet aggregation was found to be inhibited by VSRN in the doses of 1.25-5.00μg/kg and at the concentration of 6.25-50μg/L in both in vivo and in vitro test. Conclusion VSRN has powerful arteriovenous antithrombosis and antiplatelet aggregation of rats. The antithrombotic effect of VSRN is related to its platelet aggregation inhibitory activity. The above findings indicate that VSRN is an antithrombotic active ingredient of VnA.