影响桦褐孔菌培养菌丝体水解鞣质积累的因素

Factors affecting accumulation of hydrolysable tannins in cultured mycelia of Inonotus obliquus

目的探索影响人工培养条件下桦褐孔菌菌丝体水解鞣质积累的因素。方法以菌丝体干质量和水解鞣质的量为指标,对不同碳源、氮源、pH以及不同金属离子作用下的菌丝体干质量和水解鞣质的量进行测定。结果最适合桦褐孔菌积累水解鞣质的碳、氮源是葡萄糖和蛋白胨,最佳pH是5.5。Cu2+在0.8mmol/L,Co2+在1.6mmol/L,Zn2+在1.6mmol/L,Mn2+在1mmol/L,NH4+在4mmol/L时都能明显地促进水解鞣质的合成。结论以葡萄糖和蛋白胨为碳、氮源,pH为5.5的培养基有利于桦褐孔菌水解鞣质的积累。Cu2+,Co2+,Zn2+,Mn2+添加到上述培养基中是进一步提高培养产物水解鞣质积累的有效手段。

Objective To study the factors affecting the accumulation of hydrolysable tannins in cultured mycelia of Inonotus obliquus. Methods Taking dry weight of mycelia and hydrolysable tannin content as index, different carbon and nitrogen sources, pH levels, and metal ions were evaluated for the accumulation of hydrolysable tannins in the submerged culture of I. obliquus. Results The optimal carbon and nitrogen sources were glucose and peptone. Optimal initial pH value was 5.5. The accumulation of hydrolysable tannins was greatly enhanced in the medium with the presence of Cu^z＋ at 0.8 mmol/L, Co^2＋ and Zn^2＋ at 1.6 mmol/L, Mn^2＋ at 1 mmol/L, and NH4^＋ at 4 mmol/L when compared to the control. Conclusion The accumulation of hydrolysable tannins is maximized in the medium containing glucose and peptone with pH value at 5.5. Supplementation of Cu^2＋, Co^2＋, Zn^2＋, and Mn^2＋ into the medium is an effective method for further increasing the accumulation of hydrolysable tannins in cultured mycelia of I. obliquus.