单克隆抗体早期鉴别阳性钉螺效果的研究

STUDIES ON THE EARLY STAGE IDENTIFICATION OF POSITIVE SNAILS BY MONOCLONAL ANTIBODY

以多只毛蚴感染多只钉螺后，逐周取样３０只，以抗血吸虫卵的ＭＧ２ＭｃＡｂ，应用ＥＬＩＳＡ检测螺体内血吸虫抗原，并同时以镜检为对照。结果表明，感染３周阳性钉螺体内血吸虫抗原的阳性检出率为７０％，以后逐周提高，５周为９０％，８周为９６．６７％。提示该法似有早期鉴别阳性钉螺的效能。在ＥＬＩＳＡ检测不同数量毛蚴感染钉螺体内血吸虫抗原阳性率的比较中，感染后期（８周），多只毛蚴感染组螺体内抗原阳性检出率为９６．６７％；单只毛蚴感染组为１００％，两组基本相似。结果表明，不论单只毛蚴或多只毛蚴感染钉螺，在感染后期，用ＥＬＩＳＡ检测螺体内血吸虫抗原的阳性率无差异。

After snails were infected individually by exposure to many miracidia,30 of them were detected with MG 2 McAb ELISA every week for schistosome antigen and with microscopical examination for comparison. Results showed that 3 weeks after infection,positive rate of schistosome antigen in snails was 70% and increased week by week,reaching 90% 5 weeks after infection and 96.6% by the 8th week. This result suggested that the method might be used for early stage identification of positve snails. Comparison of positive rate of schistosome antigen in snails infected by different nuber of miracidia using ELISA during the late period of infection (8 weeks),antigen positive rate was 96.6% in snails infected with several miracidia,while it was 100% in the snail infected with a single miracidum. Results of both groups were basically the same. These results showed that during the late period of infection,there is no different in the positive rate of schistosome antigen in snails with 1 miracidia or with a group of miracidias ELISA was used.