2型糖尿病及胰岛素抵抗与脂蛋白脂酶基因HindⅢ和PvuⅡ酶切位点多态性相关性的研究

The Polymorphism of HindⅢ and PvuII Sites in the Lipoprotein Lipase Gene in Patients with Type 2 Diabetes Mellitus and Insulin Resistance

目的探讨脂蛋白脂酶基因第8内含子HindⅢ和第6内含子PvuⅡ酶切位点多态性与2型糖尿病及其胰岛素抵抗的发生之间是否有关联。方法选取78例2型糖尿病患者为病例组,49例正常人为正常对照组,分别对第8号内含子HindⅢ酶切位点(H)及第6号内含子PvuⅡ酶切位点(P)进行多态性分析。结果病例组等位基因"H+"和基因型H+H+分布频率分别高于正常对照组(P<0.05)。病例组中并发高甘油三酯血症组等位基因H+和基因型H+H+分布频率均高于正常甘油三酯血症患者组(P<0.05)。病例组中P+P+组高密度脂蛋白胆固醇水平低于P-P-组(P<0.05),而低密度脂蛋白胆固醇、胰岛素抵抗指数水平高于P-P-组(P<0.05)。结论脂蛋白脂酶HindⅢ酶切位点多态性与2型糖尿病及其胰岛素抵抗有关。PvuⅡ酶切位点多态性与2型糖尿病中胰岛素抵抗有关。

Objective To investigate the frequencies of variant of lipoprotein lipase （LPL） gene, HindⅢ site at intron 8 and PvuⅡ site at intron 6, and its relation with type 2 diabetes mellitus and insulin resistance （IR）. Methods PCR-RFLP method was used to determine the DNA polymorphism of 8 th intron and 6 th intron of LPL gene in 78 T2DM patients and 49 controls. Results （1） Compared with control group, the frequencies of H＋H＋ genotypes and alleles ＂H^＋＂ was significantly higher in type 2 diabetes patients （P〈0.05）. （2） In type 2 diabetes patients, the H^＋H^＋ genotype and allele H^＋ frequency of the hypertriglyeeridemia group was significantly higher than that of normal triglycerides control group （P〈0.05）. （3） The levels of LDL-C and IR in P^＋ P^＋ genotype were higher than those in P-P- genotype （P〈0.05）, but HDL-C was lower （P〈0.05）. Conclusion The polymorphism of LPL-Hind Ⅲ site is significantly associated with type 2 diabetes mellitus and insulin resistance. The polymorphism of LPL- PvuⅡ site is significantly associated with insulin resistance in type 2 diabetes.