HPLC法测定消淤止痛气雾剂中羟基红花黄色素A的含量

Determination of Hydroxyl Safflor Yellow A in Xiaoyuzhitong Qiwuji by HPLC

目的建立HPLC法测定消淤止痛气雾剂中羟基红花黄色素A含量的方法。方法色谱柱为ZORBAXSB-C18(250mm×4.6mm,5μm);流动相为甲醇-乙腈-0.7%磷酸水溶液(26∶2∶72);流速为1.0ml/min;检测波长为403nm;柱温为40℃。结果羟基红花黄色素A进样量在0.3969～2.1168μg范围内与峰面积积分值线性关系良好(r=0.9996),平均回收率为98.43%,RSD为1.81%(n=6)。结论本方法操作简单、准确、专属性强、灵敏度高、重现性好,可用于消淤止痛气雾剂的质量控制。

Aim To develop a method for determination of hydroxyl safflor yellow A in Xiaoyuzhitong Qiwuji. Methods RP-HPLC with ZORBAX SB-C18 （250mm × 4.6mm ,5μm）column was used. The mobile phase consisted of methanol-acetonitrile-0.2% acetic acid （26： 2： 72）, with a flow rate of 1.0ml/min and detection wavelength at 403nm,under 40℃. Results The linear range was 0. 396 9 -2. 116 8μg （ r =0. 999 6） ,the average recovery （ n = 6） was 98.43 % and the relative standard deviation, RSD , was 1.81%. Conclusion The method is simple , fast and applicable to the analysis of Xiaoyuzhitong Qiwuji of different sources. It can also be used for quality control of safflower preparations for clinical use.