RP-HPLC测定万通筋骨片中马兜铃酸A的含量

Determination of Aristolochic Acid A in Wantong Jingu Pian by RP-HPLC

目的建立反相高效液相色谱法测定万通筋骨片中马兜铃酸A的含量。方法色谱柱:KromasilC18(250mm×4.6mm,5μm);流动相:甲醇-0.5%冰醋酸溶液(60∶40);检测波长:250nm。结果马兜铃酸A在0.0808～0.4040μg·ml-1范围内线性关系良好,回归方程:A=129913C+68835,r=0.9999;平均回收率为99.1%,RSD为1.14%。结论本方法简便、灵敏、准确。

Aim To establish an RP-HPLC method for the determination of Aristolochic acid A in Wantong Jingu Pian. Methods The analytical column was Kromasil C18 （250mm × 4.6mm, 5μm） ;the mobile phase consisted of methanol -0.5% acetic acid of water（60： 40）, the detection wave was 250nm. Results Under this condition,there was a good linear relationship in the concentration range of 0. 080 8 - 0. 404 0μg·ml^-1. The regression equation was A = 129 913 C ＋ 688 35 （ r = 0. 999 9）. The average recovery of aristolochic acid A was 99.1% （ RSD = 1.14% ）. Conclusions This method is simple,sensitive and accurate.