HPLC法测定知母药材及其相关制剂中芒果苷和新芒果苷的含量

Determination of Mangiferin and Neomangiferin in Rhizoma Anemarrhenae and its Preparation by HPLC

目的建立一种高效液相色谱方法测定知母药材及其制剂中芒果苷和新芒果苷的含量。方法DiamonsilTMC18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水-甲酸(12∶88∶1.7);流速:0.8ml/min;检测波长:317nm。结果芒果苷和新芒果苷分别在2.45～49.00、0.99～37.60μg.ml-1范围内呈良好的线性关系(r=0.9993,r=0.9991)。方法学考察,结果均令人满意。结论本方法简便、准确、可靠,可用于知母药材及其相关制剂中芒果苷和新芒果苷的含量测定。

Aim To establish an HPLC method for the determination of mangiferin and neomangiferin in Rhizoma Anemarrhenae and its preparation. Methods The determination was performed on a Diamonsil Cls column （250 mm × 4. 6mm,5μm,Dkima） ,with the mobile phase composed of acetionitrile-water-formic acid（12： 88： 1.7）. The detection wavelength was 317nm,and the flow rate was 0.8ml/min. Results The calibration curves of mangiferin and neomangiferin were linear between 2.45 - 49.00μg·ml^- 1 （ r = 0.999 3 ）, 0. 99 - 37.60μg·ml^- 1 （ r = 0. 999 1 ）, respectively. Conclusion This method is simple, accurate, reliable and is suitable for determination of mangiferin and neomangiferin.