摘要
目的:优选珍珠菜中总皂苷提取工艺。方法:以齐墩果酸为对照品,采用紫外分光光度法测定总皂苷含量,用L9(3^4)正交设计法优化醇提工艺。结果:最优提取工艺为70%乙醇提取3次,第1次12倍量,第2次10倍量,第3次8倍量,每次1.5h。结论:优化的提取工艺简便易行,稳定可靠。
Objective: To optimize the extraction technique of total sapponin in Lysimachia clethroide Duby (LCD). Methods: Using UV spectrophotometry to determine the amount of total sapponin. The extraction technique was optimized by the orthogonal test. Results: The optimized extraction technique was to use 70% alcohol, for 3 times, 12 times of quantity (1 st time), 10 times of quantity (2 nd time)and 8 times of quantity (3 rd time)( 1.5 h per time). Conclusion: The optimized extraction technique is steady and reliable.
出处
《抗感染药学》
2007年第4期158-161,共4页
Anti-infection Pharmacy
基金
苏州市社会发展科技项目(SSY0703)
关键词
珍珠菜
皂苷
紫外分光光度法
正交法
Lysimachia clethroide duby
sapponin
UVspectrophotometry
orthogonal test