摘要
目的:研究杜仲叶桃叶珊瑚甙提取的最佳工艺条件;方法:利用响应面分析法(Response Surface Methodology)对杜仲叶中桃叶珊瑚甙的提取工艺进行优化,在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用3因素3水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以桃叶珊瑚甙提取率为响应值作响应面和等高线,在分析各个因素的显著性和交互作用后,得出杜仲叶桃叶珊瑚甙浸提的最佳工艺条件;结果:杜仲叶桃叶珊瑚甙提取的最佳工艺条件为:乙醇浓度70%,料液比1∶13,浸提温度70℃,浸提时间40min;浸提3次,杜仲叶桃叶珊瑚甙的实际提取率可达1.808%。
Objective: Optimum conditions for the extraction of aucubin from Eucommia ulmoides leaves were was studied. Method:On the basis of single factor experiments, the optimum conditions for the extraction of aucubin from Eucommia ulmoides leaves were obtained through Box- Benhnken center-united experiment design and Response Surface Methodology. Conclusion:The results showed that the optimum conditions of aucubin from Eucommia ulmoides leaves extraction were as follows:the ratio of material to solvent of 1 : 13 , ethanol concentration 70%, extraction temperature of 70 ℃ and extraction time of 40 min ,under such condition the extraction rate could be up to 1. 808% at three times extraction.
出处
《食品与机械》
CSCD
北大核心
2007年第6期55-58,121,共5页
Food and Machinery
关键词
杜仲叶
桃叶珊瑚甙
提取
响应面分析
Aucubin
Eucommia ulmoides leaves
Extraction
Response surface methodology