响应面分析法优化杜仲叶桃叶珊瑚甙提取工艺条件

Optimization of extraction technology for aucubin from eucommia ulmoides leaves via response surface methodoloy

目的:研究杜仲叶桃叶珊瑚甙提取的最佳工艺条件;方法:利用响应面分析法(Response Surface Methodology)对杜仲叶中桃叶珊瑚甙的提取工艺进行优化,在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用3因素3水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以桃叶珊瑚甙提取率为响应值作响应面和等高线,在分析各个因素的显著性和交互作用后,得出杜仲叶桃叶珊瑚甙浸提的最佳工艺条件;结果:杜仲叶桃叶珊瑚甙提取的最佳工艺条件为:乙醇浓度70%,料液比1∶13,浸提温度70℃,浸提时间40min;浸提3次,杜仲叶桃叶珊瑚甙的实际提取率可达1.808%。

Objective： Optimum conditions for the extraction of aucubin from Eucommia ulmoides leaves were was studied. Method：On the basis of single factor experiments, the optimum conditions for the extraction of aucubin from Eucommia ulmoides leaves were obtained through Box- Benhnken center-united experiment design and Response Surface Methodology. Conclusion：The results showed that the optimum conditions of aucubin from Eucommia ulmoides leaves extraction were as follows：the ratio of material to solvent of 1 ： 13 , ethanol concentration 70%, extraction temperature of 70 ℃ and extraction time of 40 min ,under such condition the extraction rate could be up to 1. 808% at three times extraction.