血管紧张素Ⅱ1型受体基因多态性与高龄原发性高血压的相关性

Relationship between polymorphism of AT_1 receptor gene and the essential hypertension in very old patients

目的研究血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与汉族高龄原发性高血压的相关关系。方法采用PCR-RFLP方法,对山东省章丘地区高龄原发性高血压患者104例(高龄高血压组)和体检正常老年人98例(正常对照组)的外周血白细胞DNA,进行AT1R基因A1166C多态性检测,观察AA、AC和CC不同基因型以及该点A、C不同等位基因频率在两组中的分布。结果AA、AC和CC基因型在高龄高血压组的分布为83(79.81%)、20(19.23%)和1(0.96%),正常对照组为82(83.67%)、16(16.33%)和0,两组比较差异无显著性意义(χ2=0.50,P>0.05);A1166与C1166等位基因频率在高龄高血压组中分别为186(89.42%)和22(10.58%),正常对照组为180(91.84%)和16(8.16%),两组比较差异无显著性意义(χ2=1.38,P>0.05)。结论AT1R基因A1166C分子变异与山东省章丘地区汉族高龄原发性高血压无相关性。

Objective To investigate the relationship between the A1166C allele polymorphism of angiotensin Ⅱ type 1 receptor（AT1R）gene and the essential hypertension in very old patients. Methods Polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） methods were used to detect the polymorphism of AT, R gene in 104 very old patients with hypertension and 98 normotensive controls. Results The genotype frequencies of A1166C were AA83 （79.81%）,AC20（19. 23%） and CCI（0.96%） in very old patients group and the corresponding frequencies were 82（83.67%）, 16（16.33%） and 0 in the control group respectively. The A1166 and C1166 allele frequencies were 186（89.42%） and 22（10.58%） respectively in very old patients group and 180（91.84%） and 16（8.16%） in normotensive group. The A1166C genotype distribution and the allele frequencies of A1166 and C1166 had no statistically significant difference in very old patients group compared with normotensive group（ P 〉0.05）. Conclusion There was no significant association between the Al166C polymorphism of AT1 R gene and the essential hypertension in very old patients in Zhangqiu of Shandong province.