血管紧张素转换酶基因多态性与老年脑卒中的相关性

Relationship between polymorphism of angiotensin converting enzyme gene and risk for stroke in elderly

目的探讨血管紧张素转换酶(ACE)基因多态性与老年脑卒中的相关性。方法选择186例脑卒中患者(脑卒中组)分为脑血栓组(126例)和脑出血组(60例),另选75例同期住院的非脑卒中患者作为对照组。采用PCR-RFLP技术,检测ACE第16内含子中长度为287bp碱基片段的插入/缺失情况,并分别测定其基因型频率和等位基因频率。结果脑卒中组D等位基因频率为41.9%,对照组为31.3%,两组比较差异有显著性意义(P<0.05);脑卒中组DD型基因频率为21.5%,对照组为9.3%,两组比较差异有显著性意义(P<0.05);脑血栓组和脑出血组D等位基因和DD型基因与对照组比较差异有显著性意义(P<0.05)。结论ACE基因多态性与老年人脑卒中相关,其DD型基因和D等位基因是老年脑卒中的危险因素。

Objective To invastigate the relationship between angiotensin converting enzyme （ACE） gene polymorphism and risk for stroke in elderly. Methods Frequencies of ACE gene type and allele were sequenced in 186 stroke patients and 75 normal controls by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） technique. The stroke patients included 126 patients with cerebral infarction and 60 patients with cerebral hemorrhage. The PCR determined the sequence located in the 16th intron with 287 bp. Results Frequency of D allele was 41.9% in stroke patients and 31.3% in the controls（ P〈0.05） and frequency of DD genotype was 21.5% in stroke patients and 9.3% in controls. The frequencies of DD genotype and D allele in cerebral hemorrhage and cerebral infarction groups were significantly higher than those in control group（ P〈0.05）. Conclusions The DD gene type of ACE and D allele may be the risk factor for stroke in elderly.