摘要
目的：观察内毒素损伤内皮细胞过程中一氧化氮（ＮＯ）的变化及其意义。方法：选择体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为研究对象，分别用内毒素及硝基左旋精氨酸（ＬＮＮＡ）对之进行处理，并检测其上清液中ＮＯ－２／ＮＯ－３丙二醛（ＭＤＡ）含量及乳酸脱氢酶（ＬＤＨ）活性变化。结果：ＬＮＮＡ可使内皮细胞ＮＯ合成降低，ＭＤＡ含量和ＬＤＨ活性下降，而ＬＰＳ组上述各项指标则明显上升。结论：内毒素可导致内皮细胞，ＮＯ增多并与氧自由基相互作用，从而引发内皮细胞损伤。
Objective: The changes of nitric oxide (NO) during cellular damage by endotoxin and its significance were studied. Methods: After the cultured human umbilical vein endothelial cells (HUVECs) were handled with endotoxin and LNNA respectively, the contents of NO-2/NO-3 and MDA and the changes of LDH activity in its supernatant were determined. Results: LNNA could inhibit the synthesis of NO and decrease the content of MDA and LDH activity. Conclusion: The overproduction of NO induced by endotoxin and the interaction between NO and oxygen free radicals might account for the damage of endothelial cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第3期202-204,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
广东自然科学基金
