杀菌性通透性增强蛋白对大肠杆菌脓毒症小鼠的保护作用

Effect of bactericidal/permeability - increasing protein on mice with E. coli sepsis

本文观察了BPI对大肠杆菌脓毒症小鼠预后的影响及其可能机制。研究结果显示,BPI治疗组动物72小时存活率明显高于生理盐水对照组(P<0.01);注菌后0.5、1h,BPI组血清内毒素水平明显低于生理盐水(NS)对照组(P<0.01);注菌后1.5h,BPI组血清TNF_α峰值也明显低于NS对照组(P<0.01);但两组间血液细菌计数在注菌后 0.5、1.5和3h均无明显差异。结果提示,BPI对大肠杆菌脓毒症具有明显的防治作用,这可能与其拮抗细菌内毒素,抑制TNF_α产生有关。

This experiment was designed to investigate effect of bactericidal/permeability - increasing protein (BPI) on the outcome of sepsis induced by E. coli J5 in mice and its possible mechanism. It was found that seventy-two hour survival rate of septic mice was significantly higher in the BPI group than that in the normal saline (NS) group (P<0.01) . At 0.5 and 1 hour following injection of bacteria respectively serum endotoxin levels in the BPI group were significantly lower than those in the NS group (P<0.01). 1.5 hours following bacterial injection the peak levels of serum TNF α in the BPI group were also markedly lower than that in the NS group (P<0.01). However, there was no significant difference in blood bacterial count between the BPI and NS groups. These results suggested that BPI had a marked protective effect on E. coli sepsis, which might be due to its antagonism against bacterial endotoxin and its inhibition of TNF a production in sepsis.