青岛市19个樱花品种的RAPD分析

RAPD Analysis on 19 Cerasus Cultivars in Qingdao

采用改良的CTAB法提取19个樱花品种的基因组DNA.利用RAPD技术对19个樱花品种进行亲缘关系鉴定和品种分类研究.从60条10 bp随机引物中筛选出32条扩增效果较好的引物进行扩增,共扩增出533条带,其中多态性带为406条,多态率达76.17%.根据扩增结果进行UPGMA聚类分析,聚类结果将19个品种分为2大类群,第1类群主要为杂交樱系;第2类群为日本晚樱系,根据樱花的重瓣性、枝姿和花色又可分为不同的亚类群、类和亚类.结果表明应用RAPD技术对樱花分子水平的分类结果与传统分类学的结果基本一致,进一步证明种源、重瓣性、枝姿和花色都可作为樱花品种分类的重要指标.

An improved CTAB method was applied to extract total genomic DNA from leaves of 19 Cerasus cultivars in Qingdao. Phylogenetic relationship identification and classification among the 19 Cerasus cultivars was studied by the technique of RAPD （Random Amplified Polymorphic DNA）. Thirty-two 10 bp primers were selected from sixty arbitrary primers. Total 533 bands were amplified, in which 406 bands were polymorphic. The percentage of polymorphic band was 76.17%. A dendrogram showing genetic relationships was constructed through UPGMA （Unweighted Pair-group Method Arithmetic Average） method. 19 Cerasus cultivars were divided into two groups. The first group included mainly hybrid cultivars; The second group was divided into several subgroups and kinds based on the characteristics of double petals, posture of branches and color of flowers. Provenance, the characteristics of double petals, posture of branches and color of flowers were the important index for classification of Cerasus cultivars. RAPD technology provides nearly the same results of classification on Cerasus cultivars to the traditional method.