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葡萄糖调节蛋白75基因对H_2O_2诱导的C6胶质瘤细胞氧化应激性损伤有保护作用 被引量：1

Protective effect of grp75 on H_2O_2-induced apoptosis of rat C6 glioma cells
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摘要 目的:研究葡萄糖调节蛋白75(grp75)基因对H2O2诱导的C6胶质瘤细胞氧化应激性损伤的保护作用。方法:以grp75基因瞬时转染C6胶质瘤细胞,以H2O2作为氧化应激刺激物,建立细胞损伤模型,运用MTT、LDH、苔盼兰拒染、细胞周期流式细胞术(FCM)和凋亡小体方法观察grp75对H2O2引起的C6细胞氧化应激性损伤的影响。结果:grp75转染组C6细胞在H2O2(0.1mmol/L,24h)刺激后MTT增加、LDH活性降低,细胞凋亡明显抑制,与空载体转染组相比有显著差异。结论:grp75基因对H2O2引起的C6细胞氧化应激性损伤有保护作用。 Objective：To investigate the protective effects of grp75 gene on hydrogen-peroxide （H2O2）-induced cell damage in rat glioma C6 cells. Methods-Cultured C6 glioma cells were transfected with grp75. Assays of MTT,LDH,FCM analysis and hoechst staining were used to investigate the protective effect of grp75 on H2O2 stimulated C6 cells. Results：Transfection of C6 glioma cells with grp75 resulted in the increased cell viability and decreased apoptosis ratio as compared to the control. Conclusion：These data indicated that grp75 might inhibit H2O2-mediated cytotoxic effect on C6 cells.
作者 张莉莉 杨柳
机构地区 南通大学护理学院 南通大学附属医院神经外科
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2007年第12期1366-1369,I0001,共5页 Journal of Nanjing Medical University(Natural Sciences)
关键词 葡萄糖调节蛋白75基因 氧化应激 C6胶质瘤细胞 grp75 oxidative stress C6 glioma cells
分类号 R363 [医药卫生—病理学]
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参考文献15

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同被引文献14

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南京医科大学学报（自然科学版）
2007年 第12期

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